摘要 |
PROBLEM TO BE SOLVED: To provide a buffer solution which, in purifying purification protein to be purified from in a sample solution by using adsorbent comprising a calcium phosphate-based compound, can suppress decomposition/degradation of the adsorbent over a long period of time and is used as elute; and a purification method for purifying purification protein from in the sample solution in which the buffer solution is used as elute.SOLUTION: The buffer solution is used as elute in selectively eluting purification protein to be purified which has been adsorbed on a carrier at least whose surface is constituted by a calcium phosphate-based compound, from the carrier. The buffer solution contains at least one of phosphoric acid and salt thereof and at least one of a compound having a carboxyl group and salt thereof. |