Use of ophthalmic compositions including lubricant, deturgescent agent, and glycosaminoglycan

摘要

Ophthalmic compositions including lubricant agent, deturgescent agent, glycosaminoglycan, and water are used to preserve tissue in vitro; for example, ocular tissue, especially a cornea, thereby facilitating subsequent transplant, especially of a complete cornea. Preferably, the ophthalmic composition and tissue are stored at about 0° C. to about 40° C. The ophthalmic solution may be replaced on either a continuous basis or batch exchanged. The volume of ophthalmic solution per individual cornea being preserved would be from about 50 ml to about 500 ml.

主权项

1. A method of preserving tissue, the method comprising the steps of:
providing excised mammalian tissue to be preserved; providing a liquid composition including glycerol in a concentration of from 0.1% to 5.0%, dextran in a concentration of from 1.0% to 10.0%, chondroitin sulfate in a concentration of from 0.1% to 5.0%, and water; and placing the excised mammalian tissue to be preserved into the liquid composition under in vitro conditions.