| 发明名称 | Method for inducing pluripotency in human somatic cells with PRDM14 or NFRKB | ||
| 摘要 | Methods of inducing pluripotency in human somatic cells and methods of maintaining pluripotency in human embryonic stem cells (hESCs) are provided, as well as cells and uses of employing such cells. The methods comprise culturing cells in the presence of (i) OCT4 and SOX2; (ii) at least one of KLF4 and c-MYC; and at least one of PRDM14 and NFRKB. | ||
| 申请公布号 | US9234180(B2) | 申请公布日期 | 2016.01.12 |
| 申请号 | US201113701437 | 申请日期 | 2011.06.02 |
| 申请人 | Agency for Science, Technology and Research | 发明人 | Ng Huck Hui;Chia Na Yu;Feng Bo |
| 分类号 | C12N5/071;C12N5/074 | 主分类号 | C12N5/071 |
| 代理机构 | Wolf, Greenfield & Sacks, P.C. | 代理人 | Wolf, Greenfield & Sacks, P.C. |
| 主权项 | 1. A method of inducing pluripotency in a human somatic cell, the method comprising: transducing the human somatic cell with one or more retroviral expression vectors encoding (i) OCT4 and SOX2; (ii) at least one of KLF4 and c-MYC; and (iii) NFRKB to thereby co-express the OCT4, SOX2, the at least one of KLF4 and c-MYC and the NFRKB in the human somatic cell; and culturing the human somatic cell in human embryonic stem cell culture medium in the presence of feeder cells to produce induced pluripotent stem cells. | ||
| 地址 | Singapore SG | ||