主权项 |
1. An isolated polynucleotide encoding a non-naturally-occurring zinc-finger binding protein comprising a non-canonical zinc finger component, wherein:
said non-canonical zinc finger component comprises the sequence X3—Cys-X2-4—Cys-X12—His-X1-7—Z—X4(SEQ ID NO:121), where X is any amino acid and Z is C, where residues 6 to 12 of X12 are engineered to bind to a target nucleic acid sequence, and wherein X1-7 is selected from the group consisting of SETG (residues 2-5 of SEQ ID NO:12), LKSLTP (residues 2-7 of SEQ ID NO:13), SEN, VRI, KRS, KRL, LRW, KRT, ITT, IKG and IGG. |