| 发明名称 | Chemically defined production of cardiomyocytes from pluripotent stem cells | ||
| 摘要 | Methods are provided for producing a cardiomyocyte population from a mammalian pluripotent stem cell population. Aspects of the methods include using a Wnt signaling agonist and antagonist, each in minimal media, to modulate Wnt signaling. Also provided are kits for practicing the methods described herein. | ||
| 申请公布号 | US9234176(B2) | 申请公布日期 | 2016.01.12 |
| 申请号 | US201314079312 | 申请日期 | 2013.11.13 |
| 申请人 | The Board of Trustees of the Leland Stanford Junior University | 发明人 | Wu Joseph;Robbins Robert C.;Burridge Paul W. |
| 分类号 | C12N5/00;C12N5/02;C12N5/071;C12N5/077 | 主分类号 | C12N5/00 |
| 代理机构 | Bozicevic, Field & Francis LLP | 代理人 | Bozicevic, Field & Francis LLP ;Sherwood Pamela |
| 主权项 | 1. A method of producing a cardiomyocyte population from a human pluripotent stem cell population, the method comprising: (a) contacting the human pluripotent stem cell population with an effective amount of a Wnt signaling agonist in a minimal media for a period of about 12-60 hours, to produce an agonist-contacted cell population; and (b) contacting the agonist-contacted cell population with an effective amount of a Wnt signaling antagonist in a minimal media for a period of at least 12 hours wherein the minimal media is selected from the group consisting of: CDM3, D3 media, D4 media, and D11 media. | ||
| 地址 | Stanford CA US | ||