Fluidic connectors and microfluidic systems

摘要

Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods amounts of time (e.g., 1 year) prior to use.

主权项

1. A method comprising:
in a fluidic device containing a first fluid, wherein the first fluid is stored in a region of the device that includes a fluid-tight seal, performing the steps of: unsealing the fluid-tight seal; introducing a sample into the fluidic device after the unsealing step by inserting a sample introduction component containing the sample into a port of the fluidic device, wherein the step of inserting the sample introduction component containing the sample into the port of the fluidic device causes the unsealing of the fluid-tight seal; passing the sample across a reaction area of the fluidic device; passing the first fluid across the reaction area; and determining a component of the sample at the reaction area.