Screening tool for anti-inflammatory drug discovery comprising the FPR2/ALX gene promoter

摘要

A screening tool for anti-inflammatory drug discovery and for the detection of the risk or presence of inflammatory conditions, comprising the sequence of the FPR2/ALX gene promoter, is disclosed.

主权项

1. A method for predicting responsiveness of a subject at risk of having or developing an inflammatory disease and/or an inflammatory event to a drug stimulating activity of an FPR2/ALX gene promoter comprising:
a) adding an active ingredient to two screening systems, a first screening system expressing a wild type FPR2/ALX promoter comprising SEQ ID NO: 3 and a second screening system expressing a FPR2/ALX promoter comprising a SNP, the sequence consisting of SEQ ID NO: 6, in parallel; b) detecting responsiveness of wild type FPR2/ALX promoter and the FPR2/ALX promoter consisting of SEQ ID NO: 6 to said active ingredient; and c) comparing expression of a reporter gene in a cell line expressing the wild type FPR2/ALX promoter of SEQ ID NO: 3 with expression of a reporter gene in a cell line expressing the FPR2/ALX promoter consisting of SEQ ID NO: 6; d) wherein the responsiveness of a subject to the active ingredient is based on the responsiveness of the wild type FPR2/ALX promoter having SEQ ID NO: 3 and the FPR2/ALX promoter consisting of SEQ ID NO: 6 to the active ingredient.