Topical composition comprising transformed bacteria expressing a compound of interest

摘要

Compositions comprised of a population of transformed bacteria formulated for topical application to a subject are described. The population of transformed bacteria are created from a non-pathogenic bacteria and transformed to express a compound of interest for a therapeutic or a cosmetic purpose. In one embodiment, the composition is for protection of the skin from ultraviolet rays.

主权项

1. A method of providing protection to human skin from UVA and UVB radiation, from 280-400 nm, by topically applying to the skin non-pathogenic, Gram-positive bacteria that has been genetically modified to express a mycosporine-like amino acid (MAA), wherein the method comprises the steps of
(a) transforming non-pathogenic, Gram-positive bacteria selected from the group consisting of Lactobacillus lactis and Lactococcus lactis with a vector comprising a sequence encoding shinorine; (b) expressing shinorine at a level of about 0.1 mM to about 100 mM for 102-1020 cfu of the transformed bacteria, and (c) topically applying to the skin of a human in need of protection from UVA and UVB radiation at least 102 cfu/cm2 of the transformed bacteria from step (a).