Microscope with reflecting fluorescence illumination optical system

摘要

A microscope includes an objective lens, an imaging lens projecting light passing through the objective lens to form an image of a specimen, an image sensor located at an imaging position where the image of the specimen is formed, an illumination light source, and a reflecting fluorescence illumination optical system including a dichroic mirror introducing light from the illumination light source into an optical to illuminate the specimen with the light. The microscope further includes a relay optical system forming an intermediate image of the specimen between the objective lens and the imaging lens to relay it to the imaging lens. The dichroic mirror of the reflecting fluorescence illumination optical system is located between the relay optical system and a pupil conjugate position that is conjugate with a pupil position of the objective lens, formed between the relay optical system and the imaging lens.

主权项

1. A microscope comprising:
an objective lens; an imaging lens projecting light passing through the objective lens to form an image of a specimen; an image sensor located at an imaging position where the image of the specimen is formed by the imaging lens; an illumination light source; a reflecting fluorescence illumination optical system including a fluorescence cube to illuminate the specimen with light, the fluorescence cube having at least a dichroic mirror that introduces light from the illumination light source into an optical path on an objective lens side and a barrier filter provided separately from the dichroic mirror in the fluorescence cube for cutting off unwanted light from the specimen; and a relay optical system arranged between the objective lens and imaging lens, the relay optical system forming an intermediate image of the specimen at a position inside the relay optical system and relaying the intermediate image to the imaging lens, wherein the fluorescence cube of the reflecting fluorescence illumination optical system is located between the relay optical system and a pupil conjugate position that is conjugate with a pupil position of the objective lens and that is formed between the relay optical system and the imaging lens, and wherein the barrier filter is located at the pupil conjugate position.