Stabilized recombinant expression plasmid vector in hafnia alvei and applications thereof

摘要

One aspect of the present disclosure relates to a stabilized recombinant expression plasmid vector comprising a polynucleotide encoding an antitoxin gene which expresses a polypeptide that neutralizes a polypeptide toxic to a host cell, the toxic polypeptide being expressed by a toxin gene in the host cell, and a polynucleotide encoding a polypeptide expression product, and the stabilized recombinant expression plasmid vector is derived from a Hafnia alvei autonomously replicable backbone plasmid. Other aspects of the present disclosure relate to a transformant transformed with the stabilized recombinant expression plasmid vector disclosed herein, a method of producing biobased cadaverine using the transformant disclosed herein, and biobased cadaverine prepared by the method disclosed herein. Another aspect of the present disclosure relates to a polyamide formed using biobased cadaverine disclosed herein, and a composition thereof. Another aspect of the present disclosure relates to a method of preparing 1,5-diisocyanatopentane comprising preparing biobased cadaverine using the method disclosed herein and converting the biobased cadaverine to 1,5-diisocyanatopentane.

主权项

1. A stabilized recombinant expression plasmid vector comprising:
a polynucleotide encoding a polypeptide that counteracts a toxin polypeptide toxic to a host cell, the toxin polypeptide being encoded by aat or abt gene, or mutants thereof, such that the toxin polypeptide has substantially the same function as the polypeptide encoded by aat or abt gene, in the host cell, a polynucleotide encoding the toxin polypeptide, and a polynucleotide encoding a polypeptide expression product, wherein: the stabilized recombinant expression plasmid vector is derived from a Hafnia alveiautonomously replicable backbone plasmid, and the host cell is Hafnia alvei, and the toxin gene and the antitoxin gene comprise a gene pair selected from the group consisting of aat/aai gene pair, abt/abi gene pair, and encoding functional fragments thereof.