Methods for fucoidan purification from seaweed extracts

摘要

Methods for purifying fucoidan in extracts from brown seaweed are disclosed. In particular, methods of purifying fucoidan in the extract to remove heavy metal ions, bacterial and endotoxin contaminants, and other impurities are disclosed. The methods include the use of a chelating agent, selective precipitation, and filtration.

主权项

1. A method for preparing a pharmaceutical composition for treating a subject in need of enhanced blood coagulation, the method comprising:
purifying a fucoidan composition from a heterogeneous mixture, wherein purifying comprises:
removing heavy metal ions from the heterogeneous mixture by treating with a chelating agent to produce a first fucoidan mixture;selectively precipitating fucoidan present in said first fucoidan mixture to remove contaminants;resuspending the fucoidan-containing precipitate in aqueous solution to produce a second fucoidan mixture; andfiltering the aqueous solution comprising fucoidan to remove bacterial and endotoxin contaminants to yield a purified fucoidan composition; and combining the purified fucoidan composition with one or more pharmaceutically acceptable excipients.