METHODS AND COMPOSITIONS FOR TAGGING AND ANALYZING SAMPLES

摘要

The invention relates to methods of tagging analytes in a sample.

主权项

1. A method of tagging target oligonucleotides comprising:
a. partitioning DNA into a plurality of compartments; b. performing an in vitro transcription reaction on the DNA within the compartments, thereby obtaining compartments comprising RNA; c. merging the interior of the compartments comprising RNA with the interior of a set of compartments comprising target oligonucleotides; d. hybridizing the RNA to the target oligonucleotides; and e. performing a reaction to attach a sequence corresponding to the RNA to the target oligonucleotides.