| 发明名称 |
METHOD FOR DETECTION OF ADENOSINE AND METABOLITES THEREOF |
| 摘要 |
This invention relates is a label-free, enzyme-free, aptamer-free method for simultaneously measuring adenosine, and its intracellular metabolites, e.g., AMP, ADP and ATP, using high pressure liquid chromatography coupled to electrochemical detector (HPLC-ECD). |
| 申请公布号 |
US2016003797(A1) |
申请公布日期 |
2016.01.07 |
| 申请号 |
US201414765649 |
申请日期 |
2014.02.28 |
| 申请人 |
ST. JUDE CHILDREN'S RESEARCH HOSPITAL |
发明人 |
Pani Amar K.;Smeyne Richard Jay |
| 分类号 |
G01N33/487;G01N27/42 |
主分类号 |
G01N33/487 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for simultaneously detecting adenosine, and its metabolites in a biological sample, comprising
(i) separating the components of a biological sample by reversed phase high pressure liquid chromatography under isocratic elution conditions; (ii) collecting fractions; and (iii) simultaneously detecting the levels of adenosine, and metabolites thereof, in each fraction with a coulometric electrochemical detector. |
| 地址 |
Memphis TN US |
