IMMUNOTHERAPEUTIC POTENTIAL OF MODIFIED LIPOOLIGOSACCHARIDES/LIPID A

摘要

Embodiments of the disclosure provide for unique lipooligosaccharide/lipid A-based mimetics for use as adjuvants. Methods of generating lipooligosaccharide/lipid A-based mimetics are provided that utilize recombinantly engineered bacteria to produce the mimetics, including, for example, addition of one or more particular enzymes such as acyltransferases, deacylases, phosphatases, or glycosyltransferases.

主权项

1. A method of generating a lipooligosaccharide/lipid A-based mimetic, comprising the steps of:
obtaining a bacterial strain that has one or more of the following modifications:
expresses one or more non-endogenous lipid A biosynthesis enzymes;expresses one or more endogenous lipid biosynthesis enzymes, wherein the enzyme is modified; and/orhas modified regulation of one or more endogenous lipid biosynthesis enzymes; and subjecting the strain to conditions suitable for production of the lipooligosaccharide/lipid A composition.