RECOMBINANT BACILLUS SUBTILIS THAT CAN GROW ON PLANT BIOMASS

摘要

In certain embodiments recombinant modified microorganisms (e.g., Gram-positive bacteria, yeasts, etc.) are provided that display on their surface a minicellulosome comprising two or more cellulolytic enzymes where the minicellulosome is self-assembled by the microorganism and resulting microorganism is capable of growing on untreated plant biomass (e.g. biomass that is not acid treated and/or enzymatically pre-digested). In certain embodiments the microorganism grows on lignocellulose as the sole carbon source.

主权项

1. A recombinant modified Gram-positive bacterium that displays on its surface a minicellulosome comprising two or more cellulolytic enzymes, wherein said bacterium comprises:
a protein encoding two or more cohesin domains wherein said protein is covalently linked to the surface of said microorganism, and wherein each of said cohesin domains is docked to a dockerin attached to a cellulolytic enzyme; and said bacterium comprises one or more constructs that encode said dockerin(s) attached to said cellulolytic enzyme(s); andwherein said minicellulosome is self-assembled by said bacterium.