DNA POLYMERASES WITH IMPROVED ACTIVITY

摘要

Disclosed are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.

主权项

1. A method of detecting and/or quantifying an RNA target comprising reverse transcribing cDNA from an RNA template with a DNA polymerase comprising an amino acid sequence at least 90% identical to the amino acid sequence of SEQ ID NO:1, wherein the amino acid of the DNA polymerase corresponding to position 522 of SEQ ID NO:1 is G.