Megakaryocyte and Platelet Production from Stem Cells

摘要

Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein.

主权项

1. A platelet production system for the ex vivo production of platelets comprising:
a fluid source, the fluid comprising a growth medium; a platelet production chamber in fluid communication with the fluid source, the chamber comprising a first and a second fluid flow path, the first and second fluid flow paths separated from each other within the chamber by a permeable scaffold; wherein the permeable scaffold is configured to allow a plurality of megakaryocytes located in the first fluid path to extend their respective proplatelet processes through the permeable scaffold toward the second fluid flow path; wherein the platelet production system is configured to transport fluid from the fluid source into the platelet production chamber; and wherein the second fluid flow path brings the growth medium into contact with at least a portion of the permeable scaffold to remove at least some platelets from the proplatelet processes.