HIGH RESOLUTION MELTING ANALYSIS ASSAY FOR THE DETECTION OF VIRAL DNA

摘要

In one aspect, the disclosure provides methods, kits and compositions for determining the presence of a JC virus mutant in a sample.

主权项

1. A method for determining the presence of a JC virus mutant in a sample, the method comprising:
amplifying nucleic acid of a JC virus in a sample in the presence of a dye that preferentially binds double-stranded nucleic acid over single stranded nucleic acid, changing the temperature of the sample and monitoring a signal corresponding to the dye binding to double-stranded nucleic acid to determine the melting temperature of double-stranded nucleic acid in the sample, and identifying the sample as comprising a JC virus mutant if the melting temperature observed for the sample is different from the melting temperature of a control sample comprising double-stranded JC virus nucleic acid of a non-mutant JC virus.