发明名称 |
IN SITU AFFINITY MATURATION OF ANTIBODIES |
摘要 |
This disclosure relates to a method to mimicking the germinal center reaction to generate antibodies with altered binding properties or increased affinity directly in hybridomas. To allow convenient and rapid affinity maturation of antibodies, a controllable activation-induced cytidine deaminase (AID) expression system to induce somatic hypermutation in hybridomas is developed. Selection of high affinity antibodies is achieved by fluorescence-activated cell sorting of hybridomas that preferentially bind fluorescence-labeled antigens. The disclosure also relates to de novo generation of hybridomas with tunable antibody affinity by generating myeloma fusion partners with controllable AID expression. |
申请公布号 |
EP2961850(A2) |
申请公布日期 |
2016.01.06 |
申请号 |
EP20140756591 |
申请日期 |
2014.02.27 |
申请人 |
ACADEMIA SINICA |
发明人 |
SU, YU-CHENG;ROFFLER, STEVE, R. |
分类号 |
C12Q1/68;C07K16/00;C07K16/40;C07K16/44;G01N33/50;G01N33/577;G01N33/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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