JAESTSTAMMAR SOM PRODUCERAR CELLULOLYTISKA ENZYMER OCH FOERFARANDE OCH MEDEL FOER UPPBYGGANDE AV DESSA.

摘要

The invention relates to a method and means for construction of yeast strains which are capable of producing cellulolytic enzymes. This is achieved by transfer of chromosomal genes or cDNA copies of mRNA which code for cellulolytic enzymes, and have been isolated from Trichoderma reesei moulds, to yeast cells by means of recombinant DNA vectors which are capable of replicating in yeasts. The correct expression of these cellulolytic genes in yeasts leads to production of cellulolytic enzymes which are secreted from the cell. This makes it possible for the yeast to hydrolyseβ- 1,4-glucan substrates such as cellulose.