Method for screening active agents that stimulate the expression of CERT to improve the skin's barrier function

摘要

A method for screening an active agent intended for preventing or combating the cutaneous signs resulting from a non-pathological impairment of barrier function, which includes the selection of active agents that stimulate the expression of the ceramide transport protein CERT in cultured human keratinocytes.

主权项

1. A method for screening active agents, which are able to increase expression of mRNA of CERT in keratinocytes from a human donor, comprising the following steps:
a) treating a sample of cultured keratinocytes from a human donor with an active agent; b) quantifying the expression of mRNA of CERT in said treated sample, relative to an untreated sample of said cultured keratinocytes which has not been treated; c) selecting the active agents that provide for an increase in the expression of mRNA of CERT relative to the untreated sample.