Analysis of electrophoretic bands in a substrate

摘要

A method and system to enhance analysis of electrophoretic bands by overlaying only the pixels of interest. The overlaid pixels are superimposed as a layer above, i.e., in the foreground of, the overlaid image, i.e., in the background. A user employs the superimposed pixels for molecular weight determination and is still able to generate densitometry analysis of the remaining pixels in the overlaid image.

主权项

1. A method for improving analysis of electrophoretic bands in a substrate, the method comprising
receiving a first image of the substrate and a second image of the substrate, the first image comprising a plurality of first electrophoretic bands captured by a first visualizing means, and the second image comprising a plurality of second electrophoretic bands captured by a second visualizing means, where the vertical position of each of the plurality of first electrophoretic bands is associated with a predefined molecular weight such that the first image represents a vertical weight scale; creating a layered composite image where the first image defines a first layer of the layered composite image and the second image defines a second layer of said layered composite image; and analyzing the layered composite image, where the analyzing step comprises a first sub-step followed by a second sub-step, where the first sub-step includes performing a molecular weight analysis by comparing information between the first image and the second image as it exists in the layered composite image, and where the second sub-step includes performing a densitometry analysis by comparing information only within the second layer.