| 发明名称 | Method for assaying OGFOD1 activity | ||
| 摘要 | The present invention relates to assays for monitoring activity of OGFOD1 activity, in particular, to assays for identifying modulators of OGFOD1 activity. The invention also relates to assays to monitor the prolyl hydroxylase activity of OGFOD1 on its substrate, the human ribosomal protein RPS23. The invention also enables the introduction of 3-hydroxyprolyl residues into peptides and proteins. | ||
| 申请公布号 | US9228224(B2) | 申请公布日期 | 2016.01.05 |
| 申请号 | US201213985243 | 申请日期 | 2012.02.10 |
| 申请人 | ISIS INNOVATION LIMITED | 发明人 | Schofield Christopher Joseph;Wolf Alexander;Ge Wei;Thalhammer Armin;Loenarz Christoph;Ratcliffe Peter John;Cockman Matthew Edward |
| 分类号 | C12Q1/26 | 主分类号 | C12Q1/26 |
| 代理机构 | Clark & Elbing LLP | 代理人 | Clark & Elbing LLP ;Michaud Susan M. |
| 主权项 | 1. A method for assaying 2-oxoglutarate and iron-dependent oxygenase domain containing 1 (OGFOD1) activity, the method comprising contacting a peptide comprising a prolyl residue, with an OGFOD1 polypeptide and determining whether the prolyl residue in said peptide is hydroxylated, wherein the OGFOD1 polypeptide comprises: (a) the amino acid sequence of SEQ ID NO: 1; (b) a variant thereof having at least 90% identity thereto and having prolyl hydroxylase activity; or (c) a fragment of either thereof having prolyl hydroxylase activity. | ||
| 地址 | Oxford GB | ||