System, method, and device for the expression or repression of proteins

摘要

This invention relates to systems, methods, and devices for inducing and/or repressing the expression of proteins. More particularly, the invention relates to systems, methods, and devices for inducing and/or repressing the expression of proteins in plastids. An exemplary embodiment involves the regulation of the expression of proteins involved in hydrogen production to stimulate the production of hydrogen gas using the methods, systems, and devices described herein.

主权项

1. A method for expressing a heterologous protein in the plastid of a cell, the method comprising the steps of
providing a Chlamydomonas cell that comprises in its plastid a construct comprising a promoter and a psbD 5′UTR operably linked to a nucleic acid encoding a heterologous protein; introducing into the nucleus of the cell a recombinant nucleic acid comprising an inducible promoter operatively linked to a nucleic acid encoding Nac2; contacting the cell with an inducer or treating the cell under conditions that result in the removal of a repressor; wherein the inducer or the repressor associates with the inducible promoter in the nucleus; wherein association of Nac2 with the mRNA encoding the heterologous protein enhances expression of the mRNA, resulting in the production of the heterologous protein in the plastid.