β-glucosidase

摘要

The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 90% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from Acremonium cellulolyticus, a polynucleotide encoding the β-glucosidase, an expression vector for expressing the β-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the β-glucosidase can be provided.

主权项

1. An isolated polypeptide comprising a protein having β-glucosidase activity and an amino acid sequence that is heterologous to the protein having β-glucosidase activity, wherein the amino acid sequence of the protein having β-glucosidase activity comprises an amino acid sequence selected from the group consisting of:
(A) the amino acid sequence set forth in SEQ ID NO: 1, (B) the amino acid sequence of SEQ ID NO: 1, except 1-20amino acids are deleted, substituted, or added and (C) an amino acid sequence having 90% or greater sequence identity with the amino acid sequence set forth in SEQ ID NO: 1, and wherein the heterologous amino acid sequence is at the N-terminal or C-terminal end of the protein having β-glucosidase activity and is selected from the group consisting of a signal peptide, a tag, a functional domain, and a linker sequence.