Method for early diagnosis of carcinomas of the anogenital tract

摘要

A method for the early diagnosis of carcinomas of the anogenital tract, preferably of the cervical carcinoma, and the preliminary stages thereof is described. The method is based on the determination of the methylation status of segments of the gene regions of ASTN1 (astrotactin 1) and ZNF671 (zinc finger protein 671, transcription factor). A DNA methylation in the promoter region or the 5′-region of these genes is indicative for carcinomas of the anogenital tract or severe preliminary stages thereof. The present invention also relates to kits which permit the conduction of the diagnostic method according to the invention.

主权项

1. A method for detecting anogenital cancer or preliminary stages thereof in a sample, comprising amplifying DNA in the sample to determine the methylation status of ASTN1 (astrotactin 1) and/or ZNF671 (zinc finger protein, transcription factor), wherein ASTN1 and/or ZNF671 are methylated in a positive sample, and wherein a primer used comprises at least one of the following sequences:(SEQ ID No.: 1)(a)CGTAAGCGTTGTTAGCGTAGC (ASTN1_F);(SEQ ID No.: 2)(b)CGCGAAATCGAAACGAAAACG (ASTN1_R);(SEQ ID No.: 11)(c)CGGAGGACGTAGTATTTATTCGC (ZNF67l_F); and(SEQ ID No.: 12)(d)CTACGTCCCCGATCGAAACG (ZNF671_R).