Process for producing an organic compound

摘要

A process of producing an organic compound and/or an intermediary compound as defined herein by feeding carbon dioxide to a culture of a cyanobacterial cell and subjecting the culture to light, wherein the cell is capable of expressing a nucleic acid molecule, wherein the expression of the nucleic acid molecule confer on the cell the ability to convert a glycolytic intermediate into the organic compound and/or into the intermediary compound and wherein the nucleic acid molecule is under the control of a regulatory system which responds to a change in the concentration of a nutrient in the culture.

主权项

1. A process for producing butanol, comprising:
culturing Cyanobacteria cells in culture medium; feeding carbon dioxide to the Cyanobacteria cells; and subjecting the Cyanobacteria cells to light, wherein,
the Cyanobacteria cells expresses a nucleic acid molecule that allows the cells to convert a pyruvate into said butanol,the nucleic acid molecule is under control of a regulatory system which responds to a change in concentration of a nutrient when culturing said Cyanobacteria cells, andsaid nucleic acid molecule encodes one or more enzyme that converts pyruvate to butanol, said enzyme being selected from the group consisting of 2-acetolactate synthetase, acetolactate decarboxylase, diacetyl reductase, acetoin reductase, glycerol dehydratase, and 1,3-propanediol dehydrogenase.