Kit for detecting a mutation and/or polymorphism of a specific region in a target nucleotide sequence

摘要

One embodiment of the disclosure provides a kit for detecting a mutation and/or polymorphism of a specific region in a target nucleotide sequence, including: at least one first primer consisting of a first segment and a second segment, wherein the first segment is a complementary strand of a first sequence and the second segment is a second sequence, and the 3′ end of the first segment connects to the 5′ end of the second segment; a second primer being a third sequence; at least one third primer consisting of a third segment and a fourth segment, wherein the third segment is a fourth sequence and the fourth segment is a complementary strand of a fifth sequence, and the 3′ end of the third segment connects to the 5′ end of the fourth segment; and a fourth primer being a complementary strand of a sixth sequence, wherein the specific region includes rs1799853, rs1057910, rs2108622, rs9923231 and rs9934438.

主权项

1. A kit for detecting a mutation and/or polymorphism of a specific region in a target nucleotide sequence, comprising: at least one first primer, a second primer, at least one third primer; and a fourth primer, wherein the at least one first primer comprises a primer whose sequence is SEQ ID NO: 16 and/or a primer whose sequence is SEQ ID NO: 17, the second primer is a primer whose sequence is SEQ ID NO: 18, the at least one third primer comprises a primer whose sequence is SEQ ID NO: 21 and/or a primer whose sequence is SEQ ID NO: 22, and the fourth primer is a primer whose sequence is SEQ ID NO: 23.