Methods and kits for detecting misfolded proteins

摘要

Methods, kits and compounds are provided that relate to the diagnosis, treatment, and/or prevention of preeclampsia.

主权项

1. A method of detecting misfolded protein aggregates in urine obtained from a pregnant woman, comprising:
(a) combining a urine sample obtained from a pregnant woman with Congo Red under conditions under which Congo Red stains proteins that are components of protein aggregates in the urine sample, thereby producing a urine sample that comprises Congo Red-stained proteins; (b) applying the urine sample that comprises Congo Red-stained proteins to a surface that has affinity to proteins, under conditions under which proteins bind to the surface and produce a Congo Red-stained surface; and (c) detecting staining intensity of the area of Congo Red-stained surface, wherein misfolded protein aggregates are detected if the staining intensity is greater than a negative control sample or comparable to a positive control sample.