SYSTEMS AND METHODS FOR RECORDING SIMULTANEOUSLY VISIBLE LIGHT IMAGE AND INFRARED LIGHT IMAGE FROM FLUOROPHORES

摘要

The invention provides systems and methods for imaging a sample. In various embodiments, the invention provides a system comprising an image sensor, a laser for emitting excitation light for an infrared or near-infrared fluorophore, a visible light source, a notch beam splitter, a notch filter, a synchronization module, an image processing unit, an image displaying unit, and light-conducting channels. In various embodiments, the present invention provides a system comprising an image sensor, a laser for emitting excitation light for an infrared or near-infrared fluorophore, a laser clean-up filter, a notch filter, a white light source, an image processing unit, an image displaying unit, and light-conducting channels. In accordance with the present invention, the image sensor can detect both visible light and infrared light.

主权项

1. An imaging system for imaging a sample comprising an infrared or near-infrared fluorophore, comprising:
a laser to emit an excitation light for the infrared or near-infrared fluorophore,
wherein the excitation light is conducted to the sample; a laser clean-up filter in the excitation light path from the laser to the sample,
wherein the laser clean-up filter narrows the wavelength band of the excitation light to the peak absorption band of the infrared or near-infrared fluorophore,wherein the narrowed excitation light excites the infrared or near-infrared fluorophore in the sample to emit an emission light,wherein the emission light is conducted to an image sensor, andwherein there is no infrared filter in the emission light path from the sample to the image sensor; a notch filter in the emission light path from the sample to the image sensor,
wherein the notch filter blocks the excitation light; and a white light source to emit a light comprising visible light,
wherein the visible light is conducted to the sample,wherein the sample reflects the visible light,wherein the reflected visible light is conducted to the image sensor, wherein the image sensor is one image sensor configured to detect both the emission light and the visible light from the sample and configured to generate sensor signals, and wherein the image sensor comprises blue, green and red pixel sensors.