| 摘要 |
Provided herein are nucleic acid tags that are linked to, or capable of linking to, a protein of interest. In particular, the nucleic acid tags are oligonucleotides comprising a reporter function and a protein tagging function. Also provided herein, are nucleic acid tag compositions, kits and methods of use thereof. |
| 主权项 |
1. A method for detecting a protein of interest that binds to a ligand, comprising:
(a) contacting the ligand, which is immobilized on a solid support container, with a fusion protein, (b) said fusion protein comprising (i) a first domain comprising the protein of interest that binds to the ligand, and (ii) a second domain comprising a nucleic acid-interacting motif, wherein the protein of interest and the nucleic acid-interacting motif differ from each other; (c) adding to said container a nucleic acid oligomer comprising (i) a first nucleic acid sequence that is a PCR amplification sequence, and (ii) a second nucleic acid sequence that binds the nucleic acid-interacting motif, wherein the first nucleic acid sequence is heterologous to the second nucleic acid sequence; (d) allowing binding in the container among the fusion protein, the ligand, and the nucleic acid oligomer; (e) removing unbound nucleic acid oligomer and unbound fusion protein; and (f) detecting whether the nucleic acid oligomer is bound to the ligand, whereby detection of bound nucleic acid oligomer indicates that the protein of interest binds to the ligand. |
