Method for Bacterial Species Identification and Strain Typing

摘要

The present disclosure describes a method for identifying a strain or species of bacteria using a single locus sequence typing technique. The single locus useful in the method is the promoter region of the 16S rRNA operon. The method is useful to identify infectious bacteria in a subject, to identifying contaminants in a food source, as well as strain typing and genetic fingerprinting of bacterial families.

主权项

1. A single locus sequence typing method for identifying an infectious bacteria in a subject having one or more infectious bacteria the method comprising,
a) obtaining a sample containing the infectious bacteria from the subject; b) analyzing a polynucleotide sequence of a promoter region of bacterial 16S rRNA operon in the infectious bacteria; and c) identifying the species and/or strain of infectious bacteria based on the sequence of the 16S rRNA promoter region.