| 主权项 |
1. A method for producing a human induced hepatocyte from a human non-hepatocyte cell, the method comprising providing a human non-hepatocyte cell, introducing into the human non-hepatocyte cell a nucleic acid or protein preparation which encodes or provides a combination of reprogramming factors, wherein the combination of reprogramming factors is selected from the group consisting of a combination comprising FOXA1, FOXA3, and HNF1A; a combination comprising FOXA1, FOXA2, and HNF1A; a combination comprising FOXA2, FOXA3, and HNF1A; a combination comprising FOXA1, HNFA1, and HNF4A; a combination comprising FOXA2, HNF1A, and HNF4A; and a combination comprising FOXA3, HNF1A, and HNF4A, and culturing the human non-hepatocyte cell under conditions suitable for reprogramming a human non-hepatocyte cell to a human induced hepatocyte, thereby producing a human induced hepatocyte from the human non-hepatocyte cell. |
