COMBINATION OF AN ALPHA-AMYLASE AND A G4-FORMING AMYLASE

摘要

This invention relates to an enzyme composition comprising an alpha-amylase polypeptide and a G4-forming amylase, a pre-mix comprising these enzymes, a method to prepare a dough and a method to prepare a baked product.;The invention also relates to methods of using the enzyme composition and the pre-mix in industrial processes, for example in food industry, such as the baking industry. The invention further relates to use of the enzyme composition or the pre-mix to reduce hardness after storage of a baked and/or to reduce loss of resilience over storage of a baked product.

主权项

1. An enzyme composition comprising an alpha-amylase polypeptide comprising
(a) an amino acid sequence as set out in amino acids 34 to 719 of SEQ ID NO: 2; or (b) an amino acid sequence having at least 99.5% identity to an amino acid sequence as set out in amino acids 34 to 719 of SEQ ID NO: 2; or (c) an amino acid sequence encoded by a polynucleotide as set out in nucleotides 100 to 2157 of SEQ ID NO: 1 or SEQ ID NO: 3; or (d) an amino acid sequence having at least 70% identity to an amino acid sequence as set out in amino acids 34 to 719 of SEQ ID NO: 2 and having at least one of Asp at position 184, Ala at position 297, Thr at position 368 and Asn at position 489, said positions being defined with reference to SEQ ID NO: 2; or (e) an amino acid sequence having at least 70% identity to an amino acid sequence as set out in amino acids 34 to 719 of SEQ ID NO: 2 and having at least one of Asp at position 184, Ala at position 297, Thr at position 368 and Asn at position 489, said positions being defined with reference to SEQ ID NO: 2 and said amino acid sequence wherein when used to prepare a baked product having a least 5 wt % sugar based on flour, said baked product has reduced hardness after storage in comparison with a baked product prepared without use of said amino acid sequence; or (f) an amino acid sequence having alpha-amylase activity and having at least 70% identity to an amino acid sequence as set out in amino acids 34 to 719 of SEQ ID NO: 2 and having a substitution, at any one or more positions corresponding to 37, 39, 46, 47, 48, 49, 53, 78, 80, 84, 87, 94, 101, 102, 103, 104, 105, 106, 107, 108, 110, 111, 113, 115, 120, 121, 127, 128, 130, 133, 136, 137, 150, 157, 158, 159, 161, 162, 163, 166, 167, 169, 176, 177, 179, 201, 207, 210, 211, 216, 219, 221, 222, 223, 227, 228, 232, 233, 234, 237, 240, 243, 247, 250, 252, 255, 258, 260, 266, 267, 268, 269, 273, 284, 285, 287, 291, 292, 293, 295, 296, 297, 299, 300, 302, 304, 306, 312, 314, 315, 316, 317, 319, 321, 332, 355, 356, 358, 360, 361, 364, 367, 383, 389, 391, 400, 403, 404, 407, 410, 411, 421, 424, 447, 454, 455, 478, 483, 500, 521, 538, 569, 581, 616, 621, 636, 670, 681, 684, 685, 693, 709, 710, said positions being defined with reference to an amino acid sequence as set out in amino acids 34 to 719 of SEQ ID NO: 2; and wherein the composition further comprises a G4-forming amylase having an amino acid sequence at least 70% identical to the amino acid sequence as set out in SEQ ID NO: 4.