DIRECT QUANTITATIVE PCR ABSENT MINOR GROOVE BINDERS

摘要

Disclosed herein are methods, compositions and kits for the quantification of a nucleic acid target present on a solid support. This entails quantitative real-time polymerase chain reaction wherein minor groove binders are excluded.

主权项

1. A method comprising combining a fluorescently labeled probe and a filter paper contacted to a specimen in a reaction vessel, performing a quantitative real-time polymerase chain reaction (qrtPCR) and detecting a level of fluorescence emanating from the reaction vessel with a charge coupled device during a thermal cycle while the paper is in the reaction vessel, wherein the probe is not conjugated with a minor groove binder and the probe is a reverse complement to a multicopy locus.