DETECTION OF TRANSLOCATION EVENTS USING GRAPHENE-BASED NANOPORE ASSEMBLIES

摘要

Translocation events are sensed using composite nanopore assemblies including nanopores formed in graphene sheets. Single molecule detection and characterization and multi-molecule characterization and identification are provided using such assemblies. Multiple electrodes associated with nanofluidic sensors facilitate detection of ionic current through a nanopore as well as tunneling currents. Current signals of individual molecules are estimated from the combination of an ionic current signal through the nanopore and tunneling current signals obtained at specific locations within the nanopore.

主权项

1. A method for detecting translocation events associated with a first target molecule while avoiding multiple binding events with respect to the first target molecule, comprising:
obtaining an assembly including a first graphene sheet bounded by first and second solid-state membranes and a nanopore extending through the graphene sheet and each of the solid-state membranes, the nanopore having an axis, the graphene sheet being positioned at a selected position with respect to the nanopore axis; bonding one or more receptors selective to the first target molecule only to the first graphene sheet; introducing an electrolyte solution to the nanopore; applying an electric potential across the nanopore, and detecting ionic current through the nanopore.