Blood velocity measurement using correlative spectrally encoded flow cytometry

摘要

A spectrally encoded flow cytometry (SEFC) technique for imaging blood in the microcirculation. Since the dependency of one of the axes of the image on time prevents effective quantification of essential clinical parameters, the optical path in an SEFC system is split into two parallel imaging lines, followed by data analysis for recovering the flow speed from the multiplexed data. The data analysis may be auto-correlation of a pair of images obtained from a sequence of images of the imaged blood vessel.

主权项

1. A method for velocity measurement of cells in a vessel, comprising:
directing a broadband illuminating beam towards said vessel; spectrally dispersing said beam in a direction across the direction of flow of said cells in said vessel; splitting said beam, in a direction generally perpendicular to the direction of the spectral dispersion of said beams, into two beams; focussing each of said beams into a separate line of illumination on said vessel, said lines being aligned generally across said vessel; collecting light reflected from said vessel along said illumination lines; spectrally decoding said reflected light to generate a sequence of two dimensional images of the motion of said cells along said vessel; and performing signal processing on doubled images of at least one of said cells, to determine the time of passage of said at least one cell between said two lines.