METHODS OF PURIFYING ANTIBODIES

摘要

Methods of purifying an antibody (Ab) from a mixture comprising impurities are disclosed. One method comprises: (a) purifying the Ab using an affinity resin; (b) purifying the Ab using a cation exchange (CEX) resin; and (c) purifying the Ab using a mixed mode (MM) resin which comprises anion exchange and hydrophobic interaction functional groups; wherein step (b) follows step (a) and step (c) follows step (b), and wherein the method does not comprise use of a hydrophobic interaction chromatography (HIC) medium, thereby purifying the Ab.