| 摘要 |
A wide-field fluorescence imaging system (210) comprises a sample platform (200), a light source (201) for illuminating a sample on the sample platform with excitation light (202), a light detector (205) having an array of sensing locations, a first filter (207) that passes wavelengths of light other than the excitation light, a second filter (206) that passes fluorescent light emitted from the sample, and a bi-telecentric optical imaging system comprising imaging optics (203a, 203b) arranged and positioned such that a first telecentric space is created or exists between the sample platform and an entry aperture stop of the bi-telecentric optical imaging system, wherein the chief rays from a plurality of field points on the sample platform are parallel to each other when passing through the first filter, and such that a second telecentric space is created or exists between the light detector and an exit aperture stop of the bi-telecentric optical imaging system, wherein the chief rays from the plurality of field points are parallel to each other when passing through the second filter. In this manner, light collected from different points in the field of view pass through the first filter at the same angles and also through the second filter at the same angles to thereby reduce or eliminate angular spectral shifting effects. |
