METHOD FOR ISOLATING NUCLEIC ACID USING MAGNETIC PARTICLE

摘要

The present invention relates to a method for isolating and purifying a nucleic acid using a magnetic particle and, more specifically, to a method for isolating a nucleic acid from a biological sample using a magnetic particle comprising the steps of: (a) adding a magnetic particle having an average particle size of 50nm - 1μm to a biological sample including a nucleic acid to thereby combine an insoluble aggregate or a nucleic acid molecule with the magnetic particle; and (b) isolating the magnetic particle combined with the insoluble aggregate or the nucleic acid molecule using a magnetic field to thereby obtain the nucleic acid. The method for isolating a nucleic acid using a magnetic particle of the present invention can dramatically reduce the time required to isolate a nucleic acid compared to a prior method and completes purification in a short time using a silica magnetic particle even in a process after a nucleic acid or protein has been isolated, thereby having an advantage of being capable of completing the whole process in a much faster time compared to centrifugation or gravity separation, etc. and also improving the yield.