发明名称 |
LIBRARY PREPARATION OF TAGGED NUCLEIC ACID USING SINGLE TUBE ADD-ON PROTOCOL |
摘要 |
A method of preparing a library of tagged nucleic acid fragments including contacting a population of cells directly with a lysis reagent having one or more protease to generate a cell lysate; inactivating the protease to generate an inactivated cell lysate, and applying a transposase and a transposon end composition containing a transferred strand to the inactivated cell lysate under conditions wherein the target nucleic acid and the transposon end composition undergo a transposition reaction. |
申请公布号 |
WO2015200609(A1) |
申请公布日期 |
2015.12.30 |
申请号 |
WO2015US37653 |
申请日期 |
2015.06.25 |
申请人 |
ILLUMINA, INC. |
发明人 |
KAPER, FIONA;CANN, GORDON |
分类号 |
C12N15/10;C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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