A METHOD FOR MAKING CHAMAECYPARIS OBUTSA OIL INHIBITING EXPRESSION OF VILURENCE FACTOR GENE OF ORAL BACTRERIA AND CHAMAECYPARIS OBUTSA OIL MADE BY THE METHOD

摘要

The present invention relates to a method for producing Chamaecyparis obtuse oil for inhibiting expression of a virulence factor gene of oral bacterial, and Chamaecyparis obtuse oil produced thereby. In particular, the method comprises the steps of: dipping a Chamaecyparis obtuse raw ingredient; feeding the Chamaecyparis obtuse raw ingredient in a pressure tank, and supplying high pressure steam and high pressure air; converting steam discharged from the pressure tank into condensed water; and separating Chamaecyparis obtuse oil and Chamaecyparis obtuse water from the condensed water. After the step of dipping the Chamaecyparis obtuse raw ingredient, a step of extracting the dipped Chamaecyparis obtuse raw ingredient by heating the same in a microwave reactor is further included in the method according to the present invention. The Chamaecyparis obtuse oil produced through the method according to the present invention has effects of inhibiting expression of virulence factor genes of oral bacteria, inhibiting proliferation of oral bacteria, inhibiting biofilm formation of oral bacteria, and inhibiting biofilm formation of oral bacteria in an artificial tooth and a natural tooth.