METHOD FOR PREPARING RECOMBINANT GLYCOPROTEINS WITH HIGH CONTENT OF SIALIC ACID BY MODULATING GLYCOSPHINGOLIPID BIOSYNTHESIS PATHWAY

摘要

The present invention relates to a method for preparing a recombinant cell line producing recombinant glycoproteins having a high sialic acid content, by inhibiting glycosphingolipid (GSL) biosynthesis pathway in the cell line producing recombinant glycoproteins. Particularly, The method of the present invention can be useful in treatment using the same, by producing erythropoietin (EPO) containing a high content of sialic acid in the cell line which induces inhibition of ceramide glucosyltransferase (CGT) by means of miRNA and siRNA specifically binding to CGT, thereby increasing an in vivo half-life of a recombinant therapeutic protein.