| 摘要 |
The invention relates to biology and medicine, specifically to the field of biochemical assays, and makes it possible to determine the presence of a ligand in a sample qualitatively or quantitatively. The essence of the invention consists in a method for determining the content of molecules of at least one type of ligand in a sample; in said method an agent or a carrier is selected, said agent or carrier having a blockable object which is not capable of specifically interacting with the detectible ligand and which participates directly or indirectly in the generation of a detectible signal, wherein spatial blocking or spatial electrostatic blocking of said blockable object governs a change in the detectible signal, on the basis of which a ligand is determined in the sample. The technical result of using the invention consists in increasing the sensitivity of the method to the detectible ligand, in reducing the assay time, in reducing the amounts of reagents required for the assay, in increasing the convenience of carrying out the assay, and in providing for the possibility of carrying out the assay in the field as well. |
