Method for creating broad-spectrum resistance to fungi in transgenic plants

摘要

The present invention relates to the creation of broad-spectrum resistance in transgenic plants by inserting inhibitory nucleic acid sequences inhibiting the expression of fungal genes.;The nucleic acid sequences of genes which are crucial to development, growth and profilation of fungi, i.e. sequences of genes with an essential function in fungi, are often conserved and/or have a high sequence identity. Regions having a particularly high sequence identity between various fungi are used in order to produce inhibitory gene constructs, e.g. on basis of antisense, siRNA, shRNA, ribozyme technology and other technologies imparting the inhibition of the expression and activation of genes. Since said methods base on the sequence-specific hybridization of the inhibitory RNA molecules with respective target sequences in certain fungal genes, the expression of all corresponding genes from various fungi is thus inhibited as well. Due to the high sequence identity of such conserved gene sections, transgenic plants are thus produced, which have broad-spectrum resistance to various fungi.

主权项

1. A method for creating broad-spectrum resistance to fungi in a transgenic plant, characterized in that a recombinant nucleic acid molecule comprising at least one nucleic acid sequence is introduced into the plant, wherein the at least one nucleic acid sequence
is identical and/or complementary to the nucleic acid sequence according to SEQ ID NO 12, and/or a part thereof comprising at least 100 nucleotides, or has a sequence identity of at least 98% to the nucleic acid sequence according to SEQ ID NO: 12,and is transcribed in the plant, so that in case of an infection of the plant with a fungus, the nucleic acid sequence, or part thereof, interacts with one or more corresponding and/or complementary nucleic acids of the fungus, such that the expression of the fungal nucleic acid sequence is reduced by at least 80%, thereby creating broad-spectrum resistance to fungi in the transgenic plant, wherein the nucleic acid sequence comprises at least 100 nucleotides and wherein the nucleic acid sequence according to SED ID NO: 12 is the conserved region within the mitochondrial ADP/ATP translocator consensus sequence.