Method and apparatus for detection, identification and quantification of single-and multi-analytes in affinity-based sensor arrays

摘要

The disclosure provides methods, device, and systems for analyzing biological array data. In particular, the disclosure provides methods and computer implemented techniques for reducing interference in microarray data, and exploiting it to obtain more accurate readouts.

主权项

1. A method of processing affinity-based array data, comprising:
(a) determining an estimate of a probe and a target interaction in an array comprising (i) analytical expression and (ii) by calibration of the array using at least one standard probe; (b) generating a stochastic-matrix that utilizes the estimate in a Markov chain model that comprises modeling hybridization, cross-hybridization, and unbound transition probabilities between states; (c) obtaining optical affinity-based array data using a light detection device; (d) applying the array data to the stochastic-matrix; (e) applying an optimization algorithm selected from the group consisting of a maximum likelihood estimation algorithm, a maximum a-posteriori criterion, a constrained least squares calculation, and any combination thereof that exploits and does not suppress non-specific interactions by considering the non-specific interactions as interference rather than noise; and (f) outputting optimized affinity-based array data to a user, wherein the optimized affinity-based array data has an improved signal-to-noise ratio compared to the array data obtained by using the light detection device; wherein at least one of the steps (d)-(f) is executed by using a suitably programmed computer.