| 发明名称 | Methods and compositions for modification of the HPRT locus | ||
| 摘要 | Nucleases and methods of using these nucleases for modification of an HPRT locus and for increasing the frequency of gene modification at a targeted locus and clones and for generating animals. | ||
| 申请公布号 | US9222105(B2) | 申请公布日期 | 2015.12.29 |
| 申请号 | US201213660821 | 申请日期 | 2012.10.25 |
| 申请人 | Sangamo BioSciences, Inc.;The Regents of the University of California | 发明人 | Cost Gregory J.;Holmes Michael C.;Kasahara Noriyuki;Laganiere Josee;Miller Jeffrey C.;Paschon David;Rebar Edward J.;Urnov Fyodor;Zhang Lei |
| 分类号 | C12N9/22;C12N15/85;C12N9/96;C12N15/90;C12N9/10 | 主分类号 | C12N9/22 |
| 代理机构 | Pasternak Patent Law | 代理人 | Pasternak Dahna S.;Pasternak Patent Law |
| 主权项 | 1. A non-naturally occurring fusion protein comprising a zinc finger protein that binds to an endogenous hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene and a nuclease cleavage domain, wherein the fusion protein modifies the endogenous HPRT gene and further wherein the zinc finger protein comprises 5 or 6 zinc finger domains, each zinc finger domain comprising a recognition helix region, wherein the zinc finger protein comprises the recognition helix regions in the order shown in a single row of rows 3 to 5 or 10 to 23 of Table 1 and further wherein the zinc finger protein targets a sequence selected from the group consisting of SEQ ID NO: 41, 42 or 125-136. | ||
| 地址 | Richmond CA US | ||