Use of brown midrib-3 gene specific markers in maize for trait introgression

摘要

This disclosure concerns compositions and methods for determining the zygosity of corn plants containing one or more brown midrib (BMR) mutations. The disclosure also concerns methods that are useful for enhancing the breeding process for BMR corn. In certain embodiments, compositions and methods for determining the zygosity of corn plants with respect to the bm3 allele.

主权项

1. A method for determining the zygosity and/or presence/absence of an allele using corn plant tissue, the method comprising:
obtaining a sample of isolated genomic DNA from the corn plant tissue; contacting the genomic DNA with at least two nucleic acid molecules capable of hybridizing to SEQ ID NO:10 under high stringency conditions, and with at least two nucleic acid molecules capable of hybridizing to SEQ ID NO:11 under high stringency conditions; amplifying the nucleotide sequence of the genomic DNA between nucleotide sequences of the genomic DNA that hybridize to the nucleic acid molecules capable of hybridizing to SEQ id NO:10 under high stringency conditions; amplifying the nucleotide sequence of the genomic DNA between nucleotide sequences of the genomic DNA that hybridize to the nucleic acid molecules capable of hybridizing to SEQ ID NO:11 under high stringency conditions; including in the amplifying reaction at least one first polynucleotide capable of hybridizing to SEQ ID NO:10 under high stringency conditions that is not capable of hybridizing to SEQ ID NO:11 under high stringency conditions, wherein the first polynucleotide is labeled with a first reporter, and including in the amplifying reaction at least one second polynucleotide capable of hybridizing to SEQ ID NO:11 under high stringency conditions that is not capable of hybridizing to SEQ ID NO:10 under high stringency conditions, wherein flee second polynucleotide is labeled with a second reporter; and detecting the levels of the first and second reporter that are hybridized to the genomic DNA, thereby determining zygosity of a bm3 mutation in the isolated genomic DNA.