| 摘要 |
FIELD: medicine.SUBSTANCE: group of inventions relates to medicine and are intended for treatment of allergy. To obtain allergen extract contact of initial material with liquid extraction means with obtaining mixture is realised. Mixture is subjected to first degree of separation. Contact of residue of initial material with means for extraction of allergens is performed. Mixture is subjected to second stage of separation with obtaining raw extract of allergen, from which low-molecular fraction is removed. The procedure is repeated until allergen extract has conductivity lower than 1000 mcSm/cm at 3-5°C with obtaining purified native allergen extract. Acidification to pH 2-4 is carried out. Then, low-molecular fraction is removed and pH is brought to 7-8 with obtaining depigmented allergen extract. Contact with glutaraldehyde is realised. Molecules with molecular size smaller than 100 kDa are removed. The procedure is repeated until allergen extract has conductivity lower than 210 mcSm/cm at 3-5°C and/or glutaraldehyde is absent. Allergen extract is a component of composition and vaccine for allergy treatment.EFFECT: application of group of inventions makes it possible to obtain allergen, possessing reduced ability to bind IgE and suitable for immunotherapy.26 cl, 10 dwg, 11 tbl, 8 ex |
