| 主权项 |
1. A method for synthesizing high fidelity target oligonucleotides, the method comprising:
a. providing an array comprising a plurality of nucleic acids, each nucleic acid having a sequence comprising a template oligonucleotide sequence and a primer binding sequence, wherein the template oligonucleotide sequence is a reverse complement of the target oligonucleotide; b. releasing the plurality of nucleic acids from the array; c. circularizing the plurality of nucleic acids; d. providing a primer, wherein the primer comprises a sequence complementary to the primer binding sequence, a restriction enzyme recognition site and self-complementary termini, under conditions promoting annealing of the primer to the primer binding sites; e. amplifying the circularized nucleic acids in rolling-circle amplification reaction using the primer thereby forming a single-stranded tandem nucleic acid sequences; f. removing the primer sequence of the single-stranded tandem nucleic acid sequences under conditions promoting hairpin formation of the primer; and g. isolating the target oligonucleotides. |
